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mcherry genes  (Addgene inc)


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    Structured Review

    Addgene inc mcherry genes
    Mcherry Genes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 2997 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px330+mcherry/pX330-U6-Chimeric_BB-CBh-hSpCas9+(Plasmid+%2342230)/pmc12796744-275-16-18
    Average 96 stars, based on 2997 article reviews
    mcherry genes - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Hierarchical Lineage Tracing Reveals Diverse Pathways of AML Treatment Resistance
    Article Snippet: Founder tag oligos were then inserted into FLARE-miniV10 via a Golden Gate Assembly reaction; the resulting product is FLARE-miniV10-10X-UMI. .. Individual Cas9 guides complementary to the target sequences in FLARE-miniV10 were designed and cloned into the pX330-mCherry (Addgene #98750) vector using the Zheng Lab pX330 cloning protocol. ..

    Article Title: Synergistic and antagonistic activities of IRF8 and FOS enhancer pairs during an immune-cell fate switch
    Article Snippet: .. To generate enhancer KO cell lines, we cloned the px330_mCherry and px330_TagBFP plasmids by inserting mCherry and TagBFP sequences (that lack a BbsI cut site), into a base px330 plasmid (Addgene #98750). .. The base px330 plasmid was a gift from Jinsong Li (Addgene plasmid # 98750; http://n2t.net/addgene:98750 ; RRID: Addgene_98750 ).

    Article Title: Synergistic and antagonistic activities of IRF8 and FOS enhancer pairs during an immune-cell fate switch.
    Article Snippet: .. Generation of enhancer knockout cell lines To generate enhancer KO cell lines, we cloned the px330_mCherry and px330_TagBFP plasmids by inserting mCherry and TagBFP sequences (that lack a BbsI cut site), into a base px330 plasmid (Addgene #98750). .. The base px330 plasmid was a gift from Jinsong Li (Addgene plasmid # 98750; http://n2t.net/addgene:98750; RRID: Addgene_98750).

    Cloning:

    Article Title: Hierarchical Lineage Tracing Reveals Diverse Pathways of AML Treatment Resistance
    Article Snippet: Founder tag oligos were then inserted into FLARE-miniV10 via a Golden Gate Assembly reaction; the resulting product is FLARE-miniV10-10X-UMI. .. Individual Cas9 guides complementary to the target sequences in FLARE-miniV10 were designed and cloned into the pX330-mCherry (Addgene #98750) vector using the Zheng Lab pX330 cloning protocol. ..

    Plasmid Preparation:

    Article Title: Transcription factor ZNF263 primes human embryonic stem cells for pluripotency dissolution and lineage commitment
    Article Snippet: The sgRNAs were designed to target Exon2 of human ZNF263 gene using Broad Institute sgRNA designer ( https://portals.broadinstitute.org/gppx/crispick/public ), and then synthesized and constructed into px330-mcherry or lentiCRISPR v2 vector (see below). .. Plasmid of px330-mcherry was kindly provided by Dr. Jinsong Li, and lentiCRISPR v2 was a gift from Feng Zhang (Addgene, plasmid #52961). .. To generate ZNF263 -knockout H1 hESCs, recombinant px330-mcherry plasmids expressing ZNF263 sgRNA were transfected to H1 single cells with Lipofectamine 3000 (Thermo Fisher Scientific, Cat#L3000015) according to the manufacturer’s protocol.

    Article Title: Synergistic and antagonistic activities of IRF8 and FOS enhancer pairs during an immune-cell fate switch
    Article Snippet: .. To generate enhancer KO cell lines, we cloned the px330_mCherry and px330_TagBFP plasmids by inserting mCherry and TagBFP sequences (that lack a BbsI cut site), into a base px330 plasmid (Addgene #98750). .. The base px330 plasmid was a gift from Jinsong Li (Addgene plasmid # 98750; http://n2t.net/addgene:98750 ; RRID: Addgene_98750 ).

    Article Title: Synergistic and antagonistic activities of IRF8 and FOS enhancer pairs during an immune-cell fate switch.
    Article Snippet: .. Generation of enhancer knockout cell lines To generate enhancer KO cell lines, we cloned the px330_mCherry and px330_TagBFP plasmids by inserting mCherry and TagBFP sequences (that lack a BbsI cut site), into a base px330 plasmid (Addgene #98750). .. The base px330 plasmid was a gift from Jinsong Li (Addgene plasmid # 98750; http://n2t.net/addgene:98750; RRID: Addgene_98750).

    Article Title: Transcription factor ZNF263 primes human embryonic stem cells for pluripotency dissolution and lineage commitment.
    Article Snippet: CRISPR-Cas9 mediated genome editing of hESCs The sgRNAs were designed to target Exon2 of human ZNF263 gene using Broad Institute sgRNA designer (https://portals.broadinstitute. org/gppx/crispick/public), and then synthesized and constructed into px330-mcherry or lentiCRISPR v2 vector (see below). .. Plasmid of px330-mcherry was kindly provided by Dr. Jinsong Li, and lentiCRISPR v2was a gift fromFengZhang (Addgene, plasmid#52961). .. To generate Nature Communications | (2025) 16:9757 13 ZNF263-knockout H1 hESCs, recombinant px330-mcherry plasmids expressing ZNF263 sgRNA were transfected to H1 single cells with Lipofectamine 3000 (Thermo Fisher Scientific, Cat#L3000015) according to themanufacturer’s protocol.

    Construct:

    Article Title: Supporting information for DPPA5A suppresses the mutagenic TLS and MMEJ pathways by modulating the cryptic splicing of Rev1 and Polq in mouse embryonic stem cells
    Article Snippet: Cas9-gRNA was constructed by introducing a guide RNA (5ʹGGCCTAGGATTCCAGAATGCTGG-3ʹ) into pX330-mcherry (Addgene,98750). .. Cas9-gRNA was constructed by introducing a guide RNA (5ʹGGCCTAGGATTCCAGAATGCTGG-3ʹ) into pX330-mcherry (Addgene,98750). ..

    Knock-Out:

    Article Title: Synergistic and antagonistic activities of IRF8 and FOS enhancer pairs during an immune-cell fate switch.
    Article Snippet: .. Generation of enhancer knockout cell lines To generate enhancer KO cell lines, we cloned the px330_mCherry and px330_TagBFP plasmids by inserting mCherry and TagBFP sequences (that lack a BbsI cut site), into a base px330 plasmid (Addgene #98750). .. The base px330 plasmid was a gift from Jinsong Li (Addgene plasmid # 98750; http://n2t.net/addgene:98750; RRID: Addgene_98750).



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